Stain Acid Fast Kit
MFR
Jorgensen
A very useful, specialized stain. Use for identification of: Johnes disease, cryptosporidium, acid fast organisms. 126ml bottle.
This 5-component kit comes with complete directions for use.
NOTE: Uses Loeffler’s Methylene Blue as a first stain. Malachite Green is not used due to hazardous shipping requirements.
| Directions | 1. Air dry and heat fix a thin film of microorganisms. Allow the slide to cool. 2. Flood the slide with Carbolfuchsin. Steam the slide with a Bunsen burner over the sink. Let the slide set for 5 minutes. Rinse with water. 3. Flood slide with Acid Alcohol for 30 seconds. Rinse with water. 4. Counterstain by flooding the slide with Methylene Blue for 30 seconds. Rinse with water. 5. Dry the slide by putting it between the pages of a book of Bibulous paper. 6. View organisms using the oil immersion objective of your microscope. |
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